回字形环山地建筑结构设计

中央美院青岛创业中心项目为角部重叠的回字形山地建筑,采用了框架-剪力墙结构体系.主体建筑结构总高度为54m,坐落在三个台地上,每两个相邻台地高差10m左右.掉层处采用"掉层脱开式且有拉梁"的方式进行结构布置.结合山地建筑结构的基本概念和受力特点,介绍了本项目的结构处理方式和设计难点,采用PKPM,MIDAS Building,SAUSAGE软件对结构进行了弹性反应谱法分析、弹性时程分析、动力弹塑性时程分析和抗震性能设计,同时针对各种不利因素采取了相应的加强措施,确保了结构安全.     

基于舰船电子设备的维修性分析与验证

舰船电子设备在作战中具有重要的作用，对舰船电子设备的维修性设计进行分析， 及时将维修性设计和验证相结合，提高舰船电子设备的维修性水平。对舰船电子设备的维修性设计进行分析和阐述，之后提出一套维修性验证程序。为舰船电子设备的维修性验证提供了新的思路。     

液相色谱-串联质谱技术在临床检验中的应用研究进展

液相色谱-串联质谱（LC-MS/MS）技术具有高灵敏度、高特异性、高分辨率和高效率的优点。近年来随着仪器灵敏度的提高，LC-MS/MS在常规临床检验中显示出极大的潜力，并在疾病早期预防和诊断中发挥着不可替代的作用。本文对LC-MS/MS在新生儿疾病筛查、维生素D检测、内分泌激素检测、肽类和蛋白质定量分析等临床检验方面的研究进展进行综述，并讨论了未来面临的挑战。     

面向草莓抓取的气动四叶片软体抓手研制

农林业中果蔬的自动化采摘需求日趋强烈,末端抓手是实现无损采摘的关键。传统的末端抓手以刚性结构居多,现有的各种柔性抓手也存在抓取力不足、包覆性不佳等缺点。本文以草莓的无损采摘为研究对象,提出将草莓外部轮廓曲线作为设计曲线,设计了一种新型气动四叶片软体抓手。首先,对软体抓手的结构做仿真优化,提出一种安全地附着在目标物表面的设想。然后,在进行草莓表面的最小破坏应力试验的基础上,测试了软体抓手的末端力,验证了其实现无损抓取的可行性。再次,利用动态捕捉技术,研究了软体抓手叶面的弯曲变形规律。最后,选择使用弧线型气体通道的软体抓手进行了草莓抓取测试,结果证明了气动四叶片软体抓手可以实现草莓的无损抓取,抓取成功率达90%,破损率为2%,表明所研制的四叶片软体抓手用于草莓抓取时具有良好的稳定性和实用性,可用于草莓采摘的末端执行器。本研究也可为其他易损果蔬的采摘技术提供理论基础和技术支撑。     

水-油连续螺旋折流板换热器热力性能研究

利用计算流体力学(Computational Fluid Dynamic, CFD)方法，针对连续螺旋折流板换热器建立物理模型和数学模型，在管侧介质为水和壳侧介质为原油条件下，研究不同原油流量及螺旋角对螺旋折流板换热器内部流场、换热性能及阻力性能的影响，并拟合了水油换热时螺旋折流板换热器的Nu、f与Re的关联式。结果表明：22°螺旋角的螺旋折流板换热器与其它较小螺旋角换热器对比，壳侧压降和换热系数逐渐减小，综合换热性能最佳。通过对壳侧原油为层流状态下的阻力系数和对流换热系数关系式进行拟合，更好地指导水-油连续螺旋折流板换热器的热力设计。     

Modified enzymatic collagen digestion-mediated isolation of osteocytes

This study established a method for isolating large numbers of high-purity osteocytes from high-density bone. Bone fragments derived from mice tibia and femurs were alternately digested with type I collagenase and EDTA nine times, and the digested cells and bone chips (BC) were cultured, digested, and passaged when cells were fully grown. The types of cells obtained were identified by morphology, viable cell counts, alkaline phosphatase staining, and biochemical activity analyses, and specific osteocyte and osteoblast markers were evaluated by quantitative real-time polymerase chain reaction. Our results showed that among the cells obtained from the third digestion (fractions 7–9) of wild mice tibias and femurs and the remaining BCs, 85%–90% of the cells were osteocytes. Moreover, their morphology was approximately one-tenth to one-fifth the size of osteoblasts, star-shaped or polygonal, with a dendritic structure, negative for alkaline phosphatase staining, and showed a high expression of dmp1 and sclerostin. Ninety percent of the cells in fractions 1–3 were osteoblasts, and were fusiform or polygonal shape. The activity of osteoblast-specific alkaline phosphatase and mRNA expression were high in this fraction, while the expression of osteocyte-specific dmp1 and sclerostin was not detected. In the second portion (fractions 4–6), a large number were osteoblasts, mixed with a small number of osteocytes, and had high alkaline phosphatase activity and osteocyte mRNA levels, a specific level of the osteocyte marker dmp1, and no sclerostin was detected. Osteocytes in daβcat^ot mice were also successfully isolated by this method, and we found that Wnt signaling increased the proliferation of these osteocytes. The proposed method can be used to culture osteocytes and osteoblasts of high purity and can be used for isolation and culture of these two kinds of cells from high-density bone, which provides an avenue for the study of osteocyte function in vitro.     

YD-1003型汽封环线切割机改进

YD-1003型汽封环线切割机存在不足,用详实的理论数据对改进加以说明,探讨了该设备的发展方向。文章采用图文并茂的对照方式来说明,比较了以往的不足及改进后达到的预期目的,提高了该型机床的工作效率,降低了事故的发生频率。     

有机絮凝剂的混凝机理研究

在混凝机理的基础上，提出了有机絮凝剂的混凝物理模型，并得到了很好地试验验证。     

RF-PCVD低温沉积无色透明类金刚石保护膜的工艺研究

采用磁约束增强射频辉光放电等离子体辅助化学气相沉积法(RF-PCVD)低温沉积出无色透明的类金刚石保护膜(DLC),主要研究了炉压P0、射频功率Pf、自生负偏压Uz、磁感应强度B、电极间距d、反应气体、镀膜时间t等工艺参数对成膜的影响.试验结果表明,外加磁场B制约了带电粒子逃逸出电极空间,提高了反应气体的离化率及等离子体浓度和活性,并使非独立变量Pf和Uz成为独立变量,有利于工艺调节.当极间距大时,需适当提高Pf,Uz和C-H流量才可得到无色、较硬的DLC膜.在比功率密度大于0.009 W·cm-2·Pa-1、C-H浓度即体积分数0.9%～1.4%及膜厚小于90nm的条件下,可沉积出无色透明、硬度较高的DLC膜.